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Rat Procollagen Ⅰ (PCⅠ) ELISA Kit

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Product Name:

Rat Procollagen Ⅰ (PCⅠ) ELISA Kit

Cat.#:

JL52525

Regulatory Status:
Alternative:

PCOL-I; PC-I; PC1; Type I Procollagen

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50ng/mL
Sensitivity:
0.36ng/mL
Specificity:
This kit recognizes Rat PCⅠ in samples. No significant cross-reactivity or interference between Rat PCⅠ and analogues was observed.
Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Procedure:
Assay Procedure
Research Area:
Metabolism
  • Rat Procollagen Ⅰ (PCⅠ) ELISA Kit
  • Rat Procollagen Ⅰ (PCⅠ) ELISA Kit
  • Rat Procollagen Ⅰ (PCⅠ) ELISA Kit
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Product Datasheets
Instruction: Down Instruction


| PCⅠ

Type I collagen is a trimer composed of two identical alpha1 (I) polypeptide chains and one α2 (I) polypeptide chain. During the extracellular transformation of procollagen into collagen, procollagen propeptides, i.e., procollagen type I carboxyl terminal propeptide (PICP) and procollagen type I amino terminal propeptide (PINP), are released into the circulation. It is a precursor synthesized by osteoblasts and secreted into the extracellular space, where it undergoes a series of post-translational modifications, including propeptides cleaved by specific proteases. The propeptides are released into the circulation, and the newly formed collagen molecules assemble into a fibrous shape.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 
Concentration (ng/mL) 50 25 12.5 6.25 3.12 1.56 0.78 0
OD 2.38 1.8 1.28 0.89 0.6 0.45 0.33 0.09
Corrected OD 2.29 1.71 1.19 0.8 0.51 0.36 0.24 -
 





| Repeatability

Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%
 

| Recovery

The recovery of Rat PCⅠ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 
Sample Type Range(%) Average Recovery(%)
Serum(n=8) 84-101 96
Plasma(n=8) 92-105 102
Cell Culture Supernates(n=8) 96-108 104





 

| Linearity

Samples were spiked with high concentrations of Rat PCⅠ and diluted with Universal Diluent to produce samples with values within the range of the assay.

 
    Serum(n=4) Plasma(n=4) Cell Culture Supernates(n=4)
1 : 2 Range (%) 84-95 88-96 90-110
Average (%) 91 93 96
1 : 4 Range (%) 89-103 87-108 105-115
Average (%) 94 98 108







 
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Sample Data
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Sample Data
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Sample Data
Size Quantity Price ($)
48T
1
360 / 360
96T
1
460 / 460
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